Cat. No.: HY-14615

[6]-Gingerol

(Synonyms: (S)-(+)-[6]Gingerol)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

[6]-Gingerol is an active compound isolated from Ginger (Zingiber officinale), exhibits a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.

For research use only. We do not sell to patients.

Purity: 99.68%
CAS No.: 23513-14-6
Formula: C₁₇H₂₆O₄
Molecular Weight:294.39

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

[6]-Gingerol Chemical Structure

CAS No.: 23513-14-6

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

[6]-Gingerol is an active compound isolated from Ginger (Zingiber officinale), exhibits a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.

IC₅₀ & Target^[2]

AMPK

Apoptosis

All AMPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	95 mg/mL Compound: Gingerol	Antiproliferative activity against human DU145 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human DU145 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
HeLa	IC₅₀	636 mM Compound: Gingerol	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 31336310]
KB	IC₅₀	480 mM Compound: Gingerol	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 31336310]
LNCaP	IC₅₀	75 mg/mL Compound: Gingerol	Antiproliferative activity against human LNCAP cells after 72 hrs by Alamar blue assay Antiproliferative activity against human LNCAP cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
LNCaP C4-2	IC₅₀	512 mg/mL Compound: Gingerol	Antiproliferative activity against human C4-2 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human C4-2 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
LNCaP C4-2B	IC₅₀	240 mg/mL Compound: Gingerol	Antiproliferative activity against human C4-2B cells after 72 hrs by Alamar blue assay Antiproliferative activity against human C4-2B cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
NCI-H1299	IC₅₀	150 μM Compound: [6]-gingerol	Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 22246389]
PC-3	IC₅₀	250 mg/mL Compound: Gingerol	Antiproliferative activity against human PC3 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human PC3 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
RBL-2H3	IC₅₀	185.1 μM Compound: 5	Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay	[PMID: 19271742]
SCC-4	IC₅₀	500 mM Compound: Gingerol	Cytotoxicity against human SCC4 cells Cytotoxicity against human SCC4 cells	[PMID: 31336310]

In Vitro

[6]-gingerol inhibits colon cancer cell proliferation and induced apoptosis, while the normal colon cells are unaffected. [6]-gingerol down-regulates phorbol myristate acetate induced phosphorylation of ERK1/2 and JNK MAP kinases and activation of AP-1 transcription factor, but has only little effects on phosphorylation of p38 MAP kinase and activation of NF-kappa B^[1]. [6]-gingerol treatment is shown to restore impaired intestinal barrier function and to suppress proinflammatory responses in DSS-treated Caco-2 monolayers. AMPK is activated on [6]-gingerol treatment^[2]. Treatment with [6]-gingerol results in a significant decrease in the viability of osteosarcoma cells in a dose-dependent fashion. In parallel, the number of cells arrested at the sub-G1 cell cycle phase is significantly increased. [6]-gingerol induces activation of caspase cascades and regulates cellular levels of Bcl2 and Bax^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In animal studies, [6]-gingerol significantly ameliorates DSS-induced colitis by restoration of body weight loss, reduction in intestinal bleeding, and prevention of colon length shortening. In addition, [6]-gingerol suppresses DSS-elevated production of proinflammatory cytokines (IL-1β, TNFα, and IL-12)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 23513-14-6
Appearance Solid
Molecular Weight 294.39
Formula C₁₇H₂₆O₄
Color White to off-white
SMILES

CCCCC[C@H](O)CC(CCC1=CC=C(O)C(OC)=C1)=O
Synonyms

(S)-(+)-[6]Gingerol
Structure Classification
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (13)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB

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Journal Impact Factor

Bioact Mater

Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy. [Abstract]

2024 Dec 10:46:82-96. PMID: 39737211
Food Chem

Conversion of gingerols to shogaols in ginger (Zingiber officinale roscoe) by puffing. [Abstract]

2024 Sep 15:452:139425. PMID: 38744128
Phytomedicine

Bioactive equivalent combinatorial components of Xiao-Xu-Ming decoction inhibit the calmodulin-mediated MLCK/MLC axis to attenuate coronary artery spasm. [Abstract]

2025 Jul:142:156713. PMID: 40349422
Free Radic Biol Med

SERCA2 dysfunction triggers hypertension by interrupting mitochondrial homeostasis and provoking oxidative stress. [Abstract]

2024 Feb 20:212:284-294. PMID: 38163553
Arch Pharm Res

Cartilage extracellular matrix regeneration with 10-gingerol via KEAP1-NRF2-ARE axis for osteoarthritis therapy. [Abstract]

2025 Dec;48(11-12):1460-1481. PMID: 41212495

[6]-Gingerol purchased from MedChemExpress. Usage Cited in: Arch Pharm Res. 2025 Dec;48(11-12):1460-1481. [Abstract]

Chondrocyte viability assessed by CCK-8 assay following treatment with [6]-Gingerol (0–100 μM).

[6]-Gingerol purchased from MedChemExpress. Usage Cited in: Arch Pharm Res. 2025 Dec;48(11-12):1460-1481. [Abstract]

A representative alricin blue staining image of glycosaminoglycans (GAGs) in chondrocytes after [6]-Gingerol treatment.

[6]-Gingerol purchased from MedChemExpress. Usage Cited in: Arch Pharm Res. 2025 Dec;48(11-12):1460-1481. [Abstract]

[6]-Gingerol treatment followed by Western blot analysis to detect the protein levels of PRG4 and COL2A1 in chondrocytes.
PLoS Biol

Molecular mechanism of the tree shrew's insensitivity to spiciness. [Abstract]

2018 Jul 12;16(7):e2004921. PMID: 30001322
J Ethnopharmacol

Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA. [Abstract]

2022 Mar 1:285:114786. PMID: 34763043
Eur J Pharmacol

6-Gingerol exerts antidepressant effect through regulating the PPARγ signaling pathway. [Abstract]

2025 Sep 15:1003:177943. PMID: 40653078
Microorganisms

The Effect of Variations in Temperature and Contact Time of Zingerone, [6]-Gingerol and Shogaol as Disinfectants on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. [Abstract]

2026 Feb 26;14(3):539. PMID: 41900299
ChemMedChem

Machine Learning-Driven Discovery of Structurally Related Natural Products as Activators of the Cardiac Calcium Pump SERCA2a. [Abstract]

2025 Jan 23:e202400913. PMID: 39853697
Appl Biochem Biotechnol

6-Gingerol Inhibits Ferroptosis in Endothelial Cells in Atherosclerosis by Activating the NRF2/HO-1 Pathway. [Abstract]

2025 Mar 22. PMID: 40120045
Kaohsiung J Med Sci

Gingerol ameliorates neuronal damage induced by hypoxia-reoxygenation via the miR-210/brain-derived neurotrophic factor axis. [Abstract]

2022 Apr;38(4):367-377. PMID: 34962339
Cell Physiol Biochem

The Effect of 6-Gingerol on Human AML Cell Lines. [Abstract]

2025 Dec 31;59(6):918-929. PMID: 41480969

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (169.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3969 mL

16.9843 mL

33.9685 mL

5 mM

0.6794 mL

3.3969 mL

6.7937 mL

10 mM

0.3397 mL

1.6984 mL

3.3969 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 25 mg/mL (84.92 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

[6]-gingerol stock (20 mg/mL) is prepared in ethanol and the working concentrations are prepared by diluting this stock in dimethyl sufoxide (DMSO). For MTT assay, 5×10³ cells/well of human colon cancer cells and 10⁴ cells/well of mouse IECs are seeded in 96-well plates. Cells are treated with [6]-gingerol for 48 h,72 h or 96 h before performing MTT assay and for 16 h before Annexin-V staining^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Mice: Mice with DSS-induced colitis are given different oral dosages of [6]-gingerol daily for 14 days. Body weight and colon inflammation are evaluated, and level of proinflammatory cytokines in colon tissues is measured^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Radhakrishnan EK, et al. [6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling. PLoS One. 2014 Aug 26;9(8):e104401. [Content Brief]

[2]. Chang KW, et al. 6-Gingerol modulates proinflammatory responses in dextran sodium sulfate (DSS)-treated Caco-2 cells and experimental colitis in mice through adenosine monophosphate-activated protein kinase (AMPK) activation. Food Funct. 2015 Oct;6(10):3334-41. [Content Brief]

[3]. Fan J, et al. 6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation. Tumour Biol. 2015 Feb;36(2):1135-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3969 mL	16.9843 mL	33.9685 mL	84.9214 mL
	5 mM	0.6794 mL	3.3969 mL	6.7937 mL	16.9843 mL
	10 mM	0.3397 mL	1.6984 mL	3.3969 mL	8.4921 mL
	15 mM	0.2265 mL	1.1323 mL	2.2646 mL	5.6614 mL
	20 mM	0.1698 mL	0.8492 mL	1.6984 mL	4.2461 mL
	25 mM	0.1359 mL	0.6794 mL	1.3587 mL	3.3969 mL
	30 mM	0.1132 mL	0.5661 mL	1.1323 mL	2.8307 mL
	40 mM	0.0849 mL	0.4246 mL	0.8492 mL	2.1230 mL
	50 mM	0.0679 mL	0.3397 mL	0.6794 mL	1.6984 mL
	60 mM	0.0566 mL	0.2831 mL	0.5661 mL	1.4154 mL
	80 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0615 mL
	100 mM	0.0340 mL	0.1698 mL	0.3397 mL	0.8492 mL

Keywords

[6]-Gingerol

13 Cited Publications

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[6]-Gingerol

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Publications Citing Use of MedChemExpress (MCE) [6]-Gingerol

All AMPK Isoforms

Biological Activity

Chemical Information

Publications (13)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

[6]-Gingerol Related Classifications

Cat. No.: HY-14615